| 09:30 - 10:30 |
Session I. Cloning and expression
|
| 1 |
Dianne Alessi: Expression of SeMet protein in insect cells
|
| 2 |
Randy Bechard: Co-expression of kinases and phosphatases
|
| 3 |
Artem Evdokimov: Methylotrophic yeast Hansenula polymorpha as an expression platform
|
| 4 |
TBD
|
10:30 - 11:00 |
Coffee break & discussion
|
11:00 - 12:00 |
Session II. Protein purification and characterization
|
| 1 |
Patrick McConnell: Seventy gallons every two weeks - at least it's not petrol!
|
| 2 |
Ken Greis: Proteomics/mass spectrometry for structural needs
|
| 3 |
Andrew Herr: Refolding proteins from bacterial inclusion bodies
|
| 4 |
TBD
|
| 12:00 - 13:30 |
Lunch, discussion, & posters
|
13:30 - 14:30 |
Session III. Protein crystallization
|
| 1 |
Tim Mueser: Solubility screening to aid crystallization
|
| 2 |
Joanne Yeh: Peptergents (tentative)
|
| 3 |
Holly Soutter: Experiments towards the crystallization of an integral membrane
protein, transhydrogenase – lessons learned
|
| 4 |
Sandra Lightle: Improvement in diffraction of ACCase CT by construct design and cryo annealing
|
| 14:30 - 15:00 |
Coffee break & discussion
|
15:30 - 16:00 |
Session IV. Structure solution & finished structures
|
| 1 |
Hideaki Moriyama: Temperature driven evolution: protein folding and stability of proteins (Laue X-ray)
|
| 2 |
Jeff Ohren: Structural basis for non-competitive inhibition of human MEK1
|
| 3 |
Eric Hnath: Phasing in the Home Laboratory
|
| 4 |
Jacqueline Vitali: Crystal structure of M. jannaschii ATCase by Molecular Replacement
|
| 16:00 - 17:00 |
Panel discussion - crystallography as a career choice in academia and industry.
|
